Applications

Bile acid is the main component of bile, and the increased concentration of bile acid in serum results from liver intake, decreased secretion or portal system shunt. For example, in acute hepatitis, chronic active hepatitis, liver cirrhosis and other diseases, TBA results will be significantly increased. Therefore, serum total bile acid (TBA) level is an important indicator of hepatic parenchymal injury. At present, it is believed that the detection of bile acid in serum is 76% sensitive and 93% specific to the diagnosis of hepatobiliary diseases. In addition, in chronic liver diseases such as liver cirrhosis, the rise of bile acid occurs earlier than the change of albumin, cholesterol, cholesterol ester, and bilirubin. Hence, it is of great significance to the diagnosis of chronic liver disease.

Principle 

The bile acid is oxidized by the 3 α-hydroxysteroid dehydrogenase and Thio-NAD+ , producing 3-ketosteroid and Thio-NADH. The amount of bile acid in serum is amplified during multiple enzyme cycles, and the thio-NADH produced can be amplified; the content of bile acid in serum can be calculated by measuring the change of Thio-NADH absorbance at 405nm.

Warnings and precautions

1.For in vitro diagnostic use.
2.Take the necessary precautions for the use of laboratory reagents.
3.Preservative contained. Do not swallow. Avoid contact with skin and mucous membranes.
4.Disposal of all waste material should be in accordance with local guidelines.
5.Material safety data sheet is available on request for professional users.