DNase I (Deoxyribonuclease I) is an endodeoxyribonuclease that can digest single- or double- stranded DNA. It recognizes and cleaves phosphodiester bonds to produce monodeoxynucleotides or single- or double- stranded oligodeoxynucleotides with phosphate groups at the 5'-terminal and hydroxyl at the 3'-terminal. The activity of DNase I depends on Ca2+ and can be activated by divalent metal ions such as Mn 2+ and Zn2+ . 5 mM Ca2+ protects the enzyme from hydrolysis. In the presence of Mg2+ , the enzyme could randomly recognize and cleave any site on any strand of DNA. In the presence of Mn2+ , the double strands of DNA can be simultaneously recognized and cleaved at almost the same site to form flat end DNA fragments or sticky end DNA fragments with 1-2 nucleotides protruding.
Product Information
| Product Name | Cat. No | Size |
| DNase I, RNase-free | HMD4007-01 | 20 μL/0.1 KU |
| HMD4007-03 | 1 mL/5 KU | |
| HMD4007-04 | 10 mL/50 KU | |
| HMD4007-05 | 840 μL/4.2 KU | |
| HMD4007-06 | 100 μL/0.5 KU |
Transportation and Storage
Storage Stability: -25℃~-15℃ for storage;
Transport Stability: Transport under ice packs;
Supplied in: 10 mM Tris-HCl, 2 mM CaCl2, 50% glycerol, pH 7.6 @25℃.
