Product Overview

LAMP Probe Master Mix (Lyophilized Beads) is a ready-to-use reagent system designed for highly efficient DNA isothermal amplification. The product contains a reaction buffer, an optimized enzyme mix including Bst DNA polymerase, and lyophilized protectant components to ensure enhanced stability and performance.

The reaction buffer is pre-formulated with Mg²⁺, dNTPs, and other essential components required for robust LAMP amplification. The reagents are lyophilized into uniform bead format, ensuring excellent consistency, convenient handling, and improved room-temperature stability during transportation and storage.

This format allows users to simply add primer-probe mix and template DNA to initiate the reaction, significantly simplifying workflow and reducing preparation time for molecular diagnostics and research applications.

Product components

Key Features

Lyophilized bead format for superior stability

Freeze-dried formulation improves reagent stability, reduces cold-chain dependence, and supports convenient storage and transportation at 2–8°C.

Ready-to-use LAMP system

Pre-mixed buffer and enzyme components eliminate the need for complex reaction setup. Only primers, probes, and template are required.

High-performance Bst DNA polymerase system

Optimized enzyme mix enables efficient strand displacement activity for rapid and sensitive isothermal amplification.

Accurate and consistent amplification results

Uniform bead formulation ensures batch-to-batch consistency and minimizes pipetting variability.

Simplified workflow for molecular diagnostics

Reduces hands-on time and improves efficiency, making it suitable for both laboratory and point-of-care (POCT) applications.

Applications

LAMP Probe Master Mix (Lyophilized Beads) is designed for DNA isothermal amplification-based applications, including but not limited to:

• Molecular diagnostic assays based on LAMP technology

• Pathogen detection and infectious disease testing

• Point-of-care testing (POCT) workflows

• Rapid nucleic acid detection in clinical and research laboratories

• Food safety and environmental monitoring

• Veterinary diagnostics

FAQ

Q1: Does the LAMP Probe Master Mix (Lyophilized Beads) contain reverse transcriptase activity?

A: No. This product contains Bst DNA polymerase with strong strand displacement activity. It does not have reverse transcriptase activity and does not support RNA-to-DNA reverse transcription.

Q2: Does this product include UDG for carryover contamination control?

A: The standard off-the-shelf lyophilized bead format does not contain UDG. However, a UDG-containing version can be customized upon request for contamination control requirements.

Q3: What are the advantages of the lyophilized bead format?

A: The lyophilized bead format provides:

• Improved reagent stability

• Reduced handling errors

• Easy transport at 2–8°C

• Ready-to-use setup (only primers and template required)
It is especially suitable for POCT and kit integration.

Q4: How long can lyophilized beads be stored at room temperature?

A: Current stability data shows approximately 3 months at room temperature. Under 2–8°C storage conditions, stability is validated for up to 12 months.

Q5: What is the difference between lyophilized beads and bottom-of-the-tube lyophilization?

A: Lyophilized beads offer flexible formats (e.g., strip tubes, vials, customized packaging), while bottom-of-the-tube lyophilization is currently limited to 8-strip tube formats. Both show comparable performance and formulation consistency.