Specification
| Appearance | Yellow-green amorphous powder， lyophilized |
| Activity | ≥5 U/mg solid |
| Cholesterol oxidase | ≤0.001% |
| Lactate oxidase | ≤0.002% |
| Uricase | ≤0.05% |
Properties
| EC number | 1.1.3.21 (Recombinant from microorganism) | |
| Molecular weight | 75kDa (SDS-PAGE) | |
| Isoelectric point | ~4.2 |
|
| Michaelis constants(L-glycerol phosphate) | K-phosphatebuffer,0.1mol/L;pH7.5:1.3 6x10–2mol/L(o-dianisidine assay) Trisbuffer,0.1mol/L;pH7.6:2.90x10–3 mol/L(o-dianisidineassay) Trisbuffer,0.1mol/L;pH8.1:1.40x10–3 mol/L(PAPassay)(L-Glycerol-3-phosphate) |
|
| Inhibitors | SDS，Ag+,Hg2+ | |
| Optimum pH | 8.0-8.5 | Fig.1 |
| Optimum temperature | 40℃ | Fig.2 |
| pH stability | pH 6.5-8.5 | Fig.3 |
| Thermal stability | Below 40℃ | Fig.4 |
Applications
This enzyme is used for the enzymatic detection of triglycerides in clinical diagnosis.
Unit definition
One unit is defined as the amount of enzyme which consumes 1 μmol of glycerol-3-P per minute at 37 ℃ under the conditions specified in the assay procedure.
