Specification
| Appearance | White amorphous powder, lyophilized |
| Purity(SDS-PAGE) | ≥90.0% |
| Enzyme powder specific activity | ≥30.0 U/mg |
| Catalase | ≤0.001% |
| uricase | ≤0.01% |
| Hexokinase | ≤0.01% |
| ATPase | ≤0.005% |
Properties
| EC number | 2.7.1.30 (Recombinant from microorganism) | |
| Molecular weight | 55 kDa (SDS-PAGE) | |
| Isoelectric point | 5 | |
| Michaelis constants | 4.4×10-5M(Glycerol),3.7×10-5M(ATP) | |
| Inhibitors | Ag+,Hg2+ | |
| Optimum pH | 7.5 | Fig.1 |
| Optimum temperature | 45℃ | Fig.2 |
| pH stability | pH 5.5-10.0 (25℃,16h) | Fig.3 |
| Thermal stability | Below 45℃ (pH7.5,30min) | Fig.4 |
| Storage stability | At least one year at-25～-15℃ | Fig.5 |
| stabilizers | BSA | |
Applications
This enzyme is used for diagnostic tests for the determination of triglycerides together with Glycerol-3-phosphate Oxidase.
Unit definition
One unit is defined as the amount of enzyme which consumes 1 μmol of glycerol per minute at 37 ℃ under the conditions specified in the assay procedure.
