Specification
| Appearance | White to pale brownish amorphous powder, lyophilized |
| Purity | ≥90% |
| Specific activity | ≥100U/mg |
| Catalase | ≤0.001% |
| Protease | ≤0.001% |
| Uricase | ≤0.01% |
Properties
| Source | Recombinant from microorganism | ||
| ECnumber | 3.1.1.13 | ||
| Molecular weight | 61kDa (SDS-PAGE) | ||
| Isoelectric point | 5.15 | ||
| Michaelis constants | 5.8×10-5M(Cholesterol linoleate) | ||
| Inhibitors | Hg2+,Ag+ | ||
| Optimum pH | 5 | Fig.1 | |
| Optimum temperature | 55℃ | Fig.2 | |
| pH stability | 4.5-9.0(37℃,60min) | Fig.3 | |
| Thermalstability | Below50℃(pH7.5,30min) | Fig. 4 | |
| Stability | Storeat-25～-15°Cfor12 months could maintain more than 90% activity | Fig.5 | |
| Protective agent | Sodium cholate and BSA | ||
Applications
Researching and mass preparation of lipoprotein cholesterol reagent in high and low density.
Unit definition
One unit of cholesterol esterase is defined as the amount of enzyme producing 1 μmol H2O2 per minute under standard determination conditions.
