Specification
| Appearance | White amorphous powder, lyophilized |
| Protein Purity | ≥90% (from SDS-PAGE) |
| Activity | ≥300 U/mg solid |
| Malate dehydrogenase |
≤0.002% |
| Lactate dehydrogenase |
≤0.002% |
| NADH oxidase | ≤0.002% |
Properties
| ECnumber | 1.1.1.30(Recombinant frommicroorganism) | |
| Molecular weight | 27kDa(SDS-PAGE) | |
| Isoelectric point | 6.1 | |
| Michaelis constants | 1.46×10-2M(D-3-Hydroxybutyrate), 9.5×10-5M(NAD+) |
|
| Inhibitors | Hg2+,Ag+,SDS | |
| Optimum pH | 8.5 | Fig. 1 |
| Optimum temperature | 60℃ | Fig.2 |
| pH stability | pH 5.0-10.0 (25℃,16h) | Fig.3 |
| Thermal stability | Below 50℃ (pH8.5,30 min) | Fig.4 |
| Storage stability | At least one year at-25～-15℃ | Fig.5 |
Applications
This enzyme is useful for enzymatic determination of ketone bodies (D-3-hydroxybutyrate and acetoacetate) in clinical analysis.
Unit definition
One unit (U) is defined as the amount of enzyme which produces 1 μmol of NADH per min under the conditions described below.
