The Lab’s Best-Kept Secret: Analyse N-Glycans in Just 2 Hours with This All-in-One Kit!

Introduction: The Essential Role of Glycan Analysis

In the dynamic world of life sciences, the understanding of N-glycans—complex carbohydrates attached to proteins—plays a vital role in therapeutic development, disease diagnostics, and immunological research. Glycoproteins, such as Immunoglobulin G (IgG), are particularly significant due to their biological activity being directly influenced by their glycosylation patterns.

To simplify and enhance this essential field, Hzymes offers a powerful solution: the HBP003120 and HBP003121 N-Glycan Analysis Kits. These kits provide researchers with a reliable, fast, and reproducible method for releasing, labeling, purifying, and analyzing N-glycans from glycoproteins.

Kit Overview: HBP003120 vs. HBP003121

Product	Samples/Kit	Ideal For
HBP003120	24 samples	Small-scale studies or pilot experiments
HBP003121	96 samples	High-throughput workflows or full-scale projects

Each kit includes four modular components:

• Glycan Release Module (includes PNGase F enzyme and buffer)
• Labeling Module (for fluorescent tagging)
• Purification Module (96-well plate-based SPE)
• Collection Module (reaction tubes, caps, collection plate, waste tray)

Step-by-Step Workflow: From Sample to Signal

1. Fast Glycan Release

The process begins by denaturing glycoproteins in a mild buffer and applying the PNGase F enzyme, which efficiently cleaves N-glycans at 50°C within 5 minutes. This rapid step is optimized for IgG but is compatible with a range of glycoproteins.

2. Fluorescent Labeling

Next, the released glycans are labeled with a proprietary fluorophore dissolved in anhydrous DMF. This step enhances the sensitivity of detection for downstream analysis and takes just 5 minutes at room temperature.

3. Solid Phase Extraction (SPE) Purification

To eliminate excess labeling reagents and isolate clean glycan profiles, the kit includes a 96-well plate system compatible with positive or negative pressure systems. A series of water, acetonitrile, and formic acid-based washes ensures high purity.

4. HILIC-FLR Analysis

Final analysis is carried out using Hydrophilic Interaction Liquid Chromatography (HILIC) with Fluorescence Detection (FLR). The suggested configuration uses:

• Column: ACQUITY UPLC® Glycan BEH Amide (2.1 x 150 mm)
• Mobile Phase A: 50 mM ammonium formate (pH 4.4)
• Mobile Phase B: 100% acetonitrile
• Detection: EX 265 / EM 425 nm

Why Choose HBP003120/HBP003121?

• Fast Results – From sample prep to analysis in under 2 hours
• High Sensitivity – Fluorescent labeling ensures excellent detection
• Modular Design – Flexible workflow with easy scalability
• Optimized for IgG – Validated with monoclonal antibodies
• Reliable Purification – SPE plates reduce background and enhance accuracy
• Friendly to All Labs – Compatible with common lab equipment

Essential Equipment Checklist

To get started, you’ll need:

• Acetonitrile (LC-MS grade), ultrapure water
• Heating module or dry bath (90°C and 50°C)
• SPE vacuum or pressure manifold
• Vortex mixer and pipettes
• Formic acid, ammonium formate buffer

Storage & Handling Tips

To ensure the best results, follow these storage conditions:

• PNGase F enzyme: -25 to -15°C, avoid repeated freeze-thaw cycles
• Labeling reagents: 2-8°C, store in the dark
• SPE plates and plasticware: Room temperature, keep dry and sealed

Conclusion: Making Glycan Science Accessible and Effective

The HBP003120 and HBP003121 N-Glycan Analysis Kits bring together speed, accuracy, and ease-of-use in one streamlined platform. Whether you are exploring therapeutic glycoproteins or advancing biomarker discovery, these kits offer a powerful yet approachable solution to N-glycan profiling.

With thoughtful design, validated reagents, and straightforward protocols, Hzymes empowers researchers to unlock the hidden world of glycans—one sample at a time.