Product Overview

BspQ I, an IIs-type restriction endonuclease, is derived from a recombinant E. coli strain that carries the cloned and modified BspQ I gene from Bacillus sphaericus.

It can recognize specific sites, and its recognition sequence and cleavage sites are as follows:

5'······GCTCTTC(N)··············3'

3'······CGAGAAG(NNNN)······5'

Key Features

Efficient and consistent digestion

Engineered to deliver robust cleavage performance with excellent batch-to-batch consistency, supporting demanding molecular biology workflows.

Precise Type IIS recognition

Recognizes its target sequence with high specificity and low off-target cleavage, enabling accurate DNA manipulation for downstream applications.

Animal-origin-free manufacturing

Produced using an animal-origin-free process and formulation, helping reduce contamination risks and supporting regulatory expectations.

GMP-ready supply

Available with GMP-compliant manufacturing support. β-lactam antibiotic-free production and DMF documentation can be provided upon request.

Applications

BspQI is widely used in workflows requiring precise DNA cleavage outside the recognition sequence, including:

• Plasmid linearization for mRNA IVT template preparation

• Golden Gate Assembly and modular cloning strategies

• Synthetic biology and DNA construct assembly

• Preparation of DNA templates for downstream enzymatic reactions

• General molecular cloning applications

Performance Data

FAQ

What applications is BspQI suitable for?

BspQI can be used in molecular cloning, Golden Gate Assembly, plasmid linearization, and DNA template preparation for mRNA IVT workflows.

Is BspQI suitable for mRNA manufacturing processes?

Yes. BspQI can be applied during plasmid linearization prior to in vitro transcription, supporting mRNA production workflows.

Is this enzyme manufactured without animal-derived components?

Yes. Both the production process and formulation are animal-origin free.

Is GMP support available?

Yes. GMP-compliant manufacturing options and DMF support are available upon request.

How should BspQI be stored and transported?

The enzyme should be transported under temperatures below 0°C and stored at -25 to -15°C.