Product Overview

BsaI is an engineered Type IIS restriction endonuclease optimized for molecular biology and mRNA manufacturing workflows. It delivers high-fidelity plasmid linearization with reduced star activity, supporting reliable downstream IVT processes.

Its recognition sequence and cleavage sites are as follows:

5'······GGTCTC(N)···············3'

3'······CCAGAG(NNNNN)······5'

Key Features

High digestion efficiency

With rapid reaction completion and excellent thermostability, ensuring reliable reaction performance.

High fidelity and strong specificity

With minimal star activity; no detectable star activity observed after 16 h incubation at 37℃.

Animal origin-free

To reduce immunogenicity risk, with fully animal-free manufacturing process and formulation.

GMP-grade availability

Free of β-lactam antibiotics, produced via GMP-compliant processes, with DMF filing available.

Applications

During the DNA template preparation stage, restriction enzymes are used to linearize the constructed plasmids for subsequent mRNA in vitro transcription (IVT).

1. Plasmid linearization for mRNA IVT template preparation

2. Golden Gate Assembly workflows

3. Synthetic biology applications

4. Molecular cloning and DNA construct preparation

FAQ

How should BsaI be stored?

Store at -25 to -15°C. Ship under ≤0°C conditions.

Is a GMP-grade version available?

Yes. GMP-compliant production and DMF support are available upon request.

Is the enzyme animal-origin free?

Yes. Both the manufacturing process and formulation are animal-origin free.

Does this enzyme exhibit star activity?

Minimal star activity was observed, with no detectable star activity after 16 h incubation at 37°C.

Can this enzyme be used for mRNA manufacturing?

Yes. It is suitable for plasmid linearization during IVT template preparation.