Product Overview

RT-LAMP Probe Master Mix (Lyophilized Beads) is a ready-to-use isothermal amplification reagent designed for rapid and highly sensitive detection of DNA and RNA targets. The product integrates Bst DNA polymerase, a thermostable reverse transcriptase (RT enzyme), reaction buffer, dNTPs, Mg²⁺, and optimized stabilizing components into a single lyophilized bead format.

After lyophilization, the reagents are pre-dispensed into stable beads, significantly simplifying workflow and improving reproducibility. Users only need to add primer/probe mix and template nucleic acid to initiate amplification, making the system highly suitable for fast molecular diagnostic applications and point-of-care testing (POCT).

The lyophilized format ensures excellent thermal stability and long-term storage at 2–8°C, supporting easy transport and global distribution without complex cold-chain requirements.

Key Features

Integrated RT-LAMP System for DNA & RNA Detection

Combines reverse transcription and LAMP amplification in a single reaction system, enabling efficient detection of both RNA and DNA targets.

Lyophilized Bead Format for High Stability

Pre-formulated freeze-dried beads enhance enzyme stability, reduce pipetting steps, and minimize contamination risk.

Ready-to-Use Workflow

Only primer/probe mix and template are required. No need for separate enzyme or buffer preparation, improving operational efficiency.

High Sensitivity and Rapid Amplification

Optimized Bst DNA polymerase and thermostable reverse transcriptase enable fast amplification with strong strand displacement activity.

Excellent Transport and Storage Stability

Stable for up to 12 months when stored at 2–8°C, suitable for distribution and field-based diagnostics.

Product components

Applications

RT-LAMP Probe Master Mix (Lyophilized Beads) is widely used in molecular diagnostics and isothermal amplification-based workflows, including:

• RNA pathogen detection (viral RNA identification)

• DNA pathogen detection (bacterial and viral DNA analysis)

• Point-of-care testing (POCT) platforms

• Rapid infectious disease screening

• Field diagnostics without full laboratory infrastructure

• Molecular assay development and kit manufacturing

FAQ

Q1: Does this RT-LAMP Probe Master Mix contain reverse transcriptase activity?

A: Yes. This product contains a heat-stable reverse transcriptase combined with Bst DNA polymerase, enabling one-step RT-LAMP amplification for both RNA and DNA targets.

Q2: What are the advantages of the lyophilized bead format?

A: The lyophilized bead format provides:

• Improved enzyme stability

• Reduced pipetting steps

• Lower contamination risk

• Better batch-to-batch consistency
It is especially suitable for POCT and ready-to-use diagnostic kits.

Q3: What do users need to add before running the reaction?

A: Only the primer/probe mix and nucleic acid template are required. No additional enzyme or buffer preparation is needed.

Q4: Can this product be used for both DNA and RNA detection?

A: Yes. The integrated RT-LAMP system supports both RNA and DNA isothermal amplification, making it suitable for pathogen detection and molecular diagnostics.

Q5: What is the storage condition and shelf life?

A: The product is transported at 2–8°C and stored at 2–8°C, with a shelf life of 12 months.

Q6: Is this product suitable for lyophilized diagnostic kit development?

A: Yes. The bead format is specifically designed for direct use in lyophilization-based assay systems, making it ideal for kit manufacturing and industrial applications.