Product Overview

RT-LAMP Probe Master Mix (Liquid, Lyoph-Ready) is a high-performance reagent designed for rapid and sensitive isothermal amplification of DNA and RNA targets. The system integrates Bst DNA polymerase, a heat-stable reverse transcriptase, reaction buffer components (Mg²⁺, dNTPs, and optimized salts), and lyophilization-compatible stabilizers.

This liquid formulation is specifically optimized for lyophilization workflows, enabling direct transition into freeze-dried (dry powder) formats when combined with suitable protectants. It is therefore ideal for kit development, industrial manufacturing, and point-of-care diagnostic product design.

For routine use, the system requires only the addition of RT-enzymes mix, primer/probe mix, and nucleic acid template, significantly simplifying assay setup while maintaining high sensitivity and amplification efficiency.

The product is supplied in a liquid format and must be stored at -25°C to -15°C, ensuring enzyme stability and consistent performance over its 12-month shelf life.

Key Features

RT-LAMP One-Step Amplification System

Combines reverse transcription and LAMP amplification in a single reaction system for both RNA and DNA detection.

Lyophilization-Ready Formulation

Optimized buffer system designed for seamless conversion into lyophilized diagnostic kits, supporting in situ freeze-drying processes.

High Sensitivity and Efficiency

Formulated with optimized Bst DNA polymerase and thermostable reverse transcriptase for rapid amplification and strong strand displacement activity.

Flexible Workflow Integration

Can be used in both liquid assays and lyophilized kit development pipelines, supporting diverse diagnostic applications.

Simplified Assay Setup

Only primer/probe mix and template are required at the point of use, reducing handling steps and minimizing contamination risk.

Stable Cold-Chain Storage

Maintains performance with storage at -25°C to -15°C and avoids repeated freeze–thaw cycles.

Applications

RT-LAMP Probe Master Mix (Liquid, Lyoph-Ready) is widely used in molecular diagnostics and assay development, including:

• RNA pathogen detection (viral RNA testing)

• DNA pathogen detection (bacterial and viral DNA analysis)

• Isothermal amplification-based diagnostic assays

• Point-of-care testing (POCT) kit development

• Lyophilized molecular diagnostic product manufacturing

• Rapid infectious disease screening

• Industrial-scale molecular diagnostic reagent production

FAQ

Q1: What does “Lyoph-Ready” mean?

A: “Lyoph-Ready” indicates that the liquid formulation is optimized for freeze-drying (lyophilization) processes, enabling direct conversion into dry reagent kits.

Q2: Does this product already contain lyophilized components?

A: No. It is a liquid master mix, but it includes stabilizers and buffer systems designed for future lyophilization compatibility.

Q3: What are the main advantages of this liquid formulation?

A: Key advantages include:

• Flexible formulation for R&D and manufacturing

• Easy integration into different LAMP systems

• High amplification efficiency

• Scalable production for industrial use

Q4: What is required to run a reaction?

A: Users need to add:

• RT-enzymes mix

• Primer/probe mix

• Nucleic acid template

Q5: What are the storage conditions?

A: The product should be stored at -25°C to -15°C and transported with dry ice. Avoid repeated freeze–thaw cycles.

Q6: What applications is this product best suited for?

A: It is ideal for:

• Molecular diagnostic kit development

• RT-LAMP assay optimization

• POCT product manufacturing

• Industrial-scale reagent production