Product Overview

LAMP Probe Master Mix (Liquid, Lyoph-Ready) is a high-performance reagent system designed for DNA isothermal amplification and molecular diagnostic applications. The product contains a complete reaction system including reaction buffer, an optimized enzyme mixes of Bst DNA polymerase, and lyophilized protectant components.

The reaction buffer is pre-formulated with Mg²⁺, dNTPs, and other essential components required for efficient Loop-mediated Isothermal Amplification (LAMP). This formulation ensures strong amplification efficiency, high sensitivity, and reliable assay performance.

This product is designed in a lyophilization-ready liquid format, allowing users to either:

• Directly mix reaction buffer, enzyme mix, and primer-probe mix for immediate use, or

• Add lyophilized protectants and connect directly to a lyophilizer for freeze-dried kit development

Only the primer-probe mix and template are required during final application, greatly simplifying workflow and improving assay convenience.

Product highlights include high amplification efficiency, strong sensitivity, and flexible compatibility for both liquid and lyophilized assay development.

Key Features

Lyophilization-ready formulation

Supports direct freeze-drying integration, enabling easy development of stable lyophilized LAMP diagnostic kits.

High-efficiency Bst DNA polymerase system

Optimized enzyme mix provides strong strand displacement activity for rapid and efficient isothermal DNA amplification.

High sensitivity and strong amplification performance

Formulated to ensure reliable detection of low-copy nucleic acid targets in molecular diagnostic applications.

Flexible usage formats

Can be used as a liquid master mix or further processed into lyophilized form depending on application needs.

Simplified workflow

Only primer-probe mix and template are required for final assay setup, reducing preparation time and operational complexity.

Stable and robust reaction system

Pre-optimized buffer system containing Mg²⁺ and dNTPs ensures consistent amplification performance.

Applications

LAMP Probe Master Mix (Liquid, Lyoph-Ready) is suitable for a wide range of DNA isothermal amplification applications, including:

• Molecular diagnostic assay development

• Pathogen detection and infectious disease testing

• Point-of-care testing (POCT) system development

• Lyophilized LAMP kit manufacturing

• Food safety and contamination detection

• Veterinary infectious disease diagnostics

• Environmental microbial detection

FAQ

Q1: What does “lyophilization-ready” mean for this product?

A: It means the liquid master mix is pre-optimized for freeze-drying. Users can directly mix it with lyophilization protectants and connect to a lyophilizer to produce stable dried LAMP kits.

Q2: Does this product contain reverse transcriptase activity?

A: No. This product contains Bst DNA polymerase with strand displacement activity but does not include reverse transcriptase function.

Q3: Can this product be used for both liquid assays and lyophilized kits?

A: Yes. It is designed as a flexible system that supports:

• Direct liquid LAMP reactions

• Development of lyophilized diagnostic kits

Q4: Does it contain UDG for contamination prevention?

A: Standard formulation does not include UDG. A UDG-containing system can be provided upon request depending on assay requirements.

Q5: How can sensitivity be improved in this system?

A: Sensitivity can be optimized by:

• Increasing Bst enzyme concentration

• Adjusting primer concentration

• Selecting high-efficiency enzyme variants (e.g., Bst V2.0)
Optimization should be balanced to avoid non-specific amplification.