Product Overview

LAMP Fluorescent Master Mix (Liquid, Lyoph-Ready) is a high-performance reagent system designed for flexible loop-mediated isothermal amplification (LAMP) workflows and lyophilization-compatible assay development. The product contains a reaction buffer, Bst DNA polymerase enzyme mix, lyophilization protectants, and fluorescent dye components.

The reaction buffer is pre-formulated with Mg²⁺, dNTPs, and other essential components required for efficient DNA amplification. Users can directly combine the reaction buffer, enzyme mix, fluorescent dye, and primers before adding template DNA for amplification.

When needed, the lyophilization protectant can be incorporated to enable the preparation of a lyophilizable LAMP system, supporting assay conversion from liquid formulation to freeze-dried format without performance loss. This makes the product suitable for both research applications and diagnostic assay development requiring flexible formulation formats.

Key Features

Lyoph-Ready formulation:

Designed to support direct conversion into lyophilized (freeze-dried) LAMP systems when needed.

High sensitivity and robustness:

Enables reliable detection even at low template concentrations.

Strong inhibitor tolerance:

Performs well in the presence of common PCR/LAMP inhibitors from crude samples.

High specificity:

Demonstrates minimal non-specific amplification, with no NTC (no-template control) amplification observed under optimized conditions.

Compatible with dUTP/UDG system:

Supports carryover contamination prevention workflows.

Flexible workflow design:

Users can customize reaction setup by combining buffer, enzyme mix, dye, and primers as needed.

Consistent performance after lyophilization:

Maintains amplification efficiency and fluorescence signal stability in freeze-dried formats.

Applications

• DNA isothermal amplification (LAMP-based assays)

• Molecular diagnostic assay development

• Point-of-care testing (POCT) workflow design

• Pathogen nucleic acid detection

• Development of lyophilized molecular diagnostic kits

• Research in rapid DNA amplification and detection systems

FAQ

Q1: What does “Lyoph-Ready” mean?

A: “Lyoph-Ready” indicates that the liquid formulation can be directly converted into a lyophilized (freeze-dried) system by adding lyophilization protectants, without affecting amplification performance.

Q2: Does this product already contain all LAMP components?

A: The system contains reaction buffer, Bst DNA polymerase enzyme mix, fluorescent dye, and optional lyophilization protectants. Users can assemble the full reaction system by mixing buffer, enzyme, dye, and primers.

Q3: Can this product be used for both liquid and lyophilized LAMP systems?

A: Yes. It is designed for flexible workflow use, supporting both liquid LAMP reactions and development of freeze-dried diagnostic kits.

Q4: How does this product perform in inhibitor-rich samples?

A: It shows strong inhibitor tolerance and maintains amplification efficiency even in crude or partially purified sample matrices.

Q5: Does it support contamination control systems such as UDG/dUTP?

A: Yes. The system is compatible with dUTP/UDG-based carryover contamination prevention workflows.

Q6: What are the performance advantages of this master mix?

A: It offers high sensitivity, strong specificity (no NTC amplification under optimized conditions), and consistent performance after lyophilization.