In the rapidly advancing field of mRNA technology, nucleoside triphosphates (NTPs)—both natural and modified—are indispensable building blocks for in vitro transcription (IVT) and mRNA drug development. At Hzymes Biotech, we provide a full range of GMP-grade NTPs, designed for optimal performance in therapeutic, diagnostic, and research applications.

What Are NTPs?

Natural NTPs

Natural nucleotides are the core substrates used in RNA synthesis and enzymatic reactions. Hzymes offers the following high-purity, GMP-grade natural NTPs:

• ATP sodium solution 

• GTP sodium solution 

• CTP sodium solution 

• UTP sodium solution

These NTPs are essential for PCR, IVT, RT-PCR, and NGS workflows.

Modified NTPs

Modified NTPs enhance the properties of synthetic mRNA by improving stability, translation, and immune tolerance. Hzymes supplies two key modified nucleotides:

• Pseudo UTP sodium solution (Pseudouridine Triphosphate) 

• N1-Me-Pseudo UTP sodium solution (N1-Methylpseudouridine Triphosphate)

These are critical for improving the safety, efficacy, and longevity of mRNA therapeutics.

Key Benefits of Modified NTPs in mRNA Therapeutics

1. Reduced Immunogenicity

Modified NTPs like pseudouridine and N1-methylpseudouridine reduce immune recognition, resulting in lower inflammatory responses and better tolerability of mRNA drugs.

2. Enhanced Stability

Incorporating modified nucleotides helps mRNA resist degradation by nucleases, extending cellular half-life and improving protein expression yield.

3. Improved Translation Efficiency

N1-methylpseudouridine in particular is known to increase protein production, outperforming even pseudouridine in certain systems.

4. Clinical Validation

Both Pfizer-BioNTech and Moderna use N1-Me-Pseudo UTP in their COVID-19 vaccines, demonstrating the real-world effectiveness of this modification in boosting therapeutic performance.

Example of common natural and modified nucleotides in mRNA therapy. All the lVT reaction data in this brochure was generated by the nucleotides list above.

Why Choose Hzymes for Your NTP Needs?

✅ GMP-Grade Production

• Manufactured under strict GMP conditions
• FDA DMF available
• Suitable for clinical and commercial applications

✅ High Purity, Low Impurity

• Ultra-low metal ion contamination
• HPLC and NMR verified purity

✅ Full Regulatory Support

• Batch-to-batch consistency
• COA and regulatory documents provided

Applications

• In vitro transcription for mRNA vaccines and therapeutics
• Synthetic biology and gene therapy
• RNA probe synthesis
• CRISPR guide RNA production
• Research reagent kits

Explore Hzymes’ NTP Portfolio

Conclusion

As the field of mRNA drug development expands, choosing the right nucleotide raw materials becomes crucial. Hzymes' full line of GMP-grade natural and modified NTPs empowers researchers and manufacturers to build better, safer, and more effective mRNA-based products.