Background

Infectious diseases are common in medical institutions, especially during high-incidence seasons. Recently, the incidence and patient visits related to chikungunya fever have significantly increased in Guangdong Province, China. However, primary healthcare institutions are overwhelmed due to inadequate infrastructure: a surge in patients but difficulty identifying pathogens, poor conditions exacerbating cross-infections, and traditional nucleic acid testing being slow and dependent on cold-chain logistics.

Overcoming the technical barriers of rapid detection and room-temperature transport has become a critical challenge in improving diagnostic efficiency at the grassroots level and optimizing the allocation of medical resources.

Featured Product

Fast, Resistant, Lyophilized PCR MIX (HMD3916)

To address challenges such as slow turnaround in grassroots nucleic acid testing labs and the need for cold storage of reagents, Hzymes has launched a “Fast + Interference-Resistant + Lyophilized PCR MIX” solution:

Pathogen Identified in 20 Minutes: RNA detection in as fast as 20 minutes; from sampling to result is significantly accelerated.

Interference-Resistant Champion: Compatible with sample release agents for direct amplification after lysis, offering excellent resistance to inhibitors.

Lyophilization Tech Breakthrough: Stable for 60 days at 50°C – no cold chain needed, making distribution to primary sites stress-free.

Simplicity Redefined: Fully reconstituted lyophilized beads + one-step sample addition = ultra-simplified operation.

PCR MIX (HMD3916) Test Data

01 Ultra-Fast: RNA Detection in 20 Minutes

Test Method: Different respiratory pathogens were tested using both fast and ultra-fast programs to evaluate reagent performance.

HMD3916 showed consistent Ct values (<0.2 Ct difference) between conventional and fast programs. The ultra-fast program differed by only about 0.5 Ct, enabling RNA detection within 20 minutes.

02 Interference Resistance: Direct Amplification with Sample Release Agent

Test Method: Influenza A and B virus controls were diluted to the same concentration using TE buffer, HMD3508 release agent, and the same solutions with oral swab-derived inhibitors. Direct amplification was performed for each condition.

HMD3916, when used with the HMD3508 release agent, demonstrated strong performance under inhibitor-rich conditions, confirming excellent resistance.

03 Stability: Lyophilized Beads Stable for 60 Days at 50°C

HMD3916A lyophilized beads retained good form and stable reagent performance after 60 days of accelerated storage at 50°C.

 
Performance and Stability Evaluation of Sample Release Agent

01 Evaluation with Throat Swab Samples

HMD3508 demonstrated superior release efficiency with throat swab samples.

02 Evaluation with Simulated Blood Samples

HMD3508 showed better resistance and release performance with simulated blood samples.

03 Room Temperature Stability of Release Agent

Testing after 3 and 6 hours at room temperature showed that HMD3508 maintained better performance even after 6 hours.

04 Stability at 2–8°C

After storage at 2–8°C for 24, 40, and 48 hours, HMD3508 still delivered stable performance, even after 48 hours.

Product Information

One-Stop Lyophilized PCR Solution