In the ever-evolving world of molecular biology, time, accuracy, and reliability are essential. That’s why the One-Step Multiplex RT-qPCR Master Mix (ONE TUBE) by Hzymes is more than just a lab reagent—it’s a trusted companion in every scientist’s journey. This all-in-one RT-qPCR kit is designed to simplify complex workflows, reduce contamination risks, and support researchers in achieving accurate RNA detection faster and with greater peace of mind.

What is One-Step Multiplex RT-qPCR Master Mix?

The One-Step Multiplex RT-qPCR Master Mix is a powerful, ready-to-use reagent mix that allows both reverse transcription and real-time quantitative PCR (qPCR) to happen in a single tube. Whether you are performing singleplex or multiplex assays, this mix ensures consistency and reduces handling steps.

With its easy-to-use format and high-performance enzyme components, the mix is tailored for labs that demand both speed and accuracy in RNA detection.

Why Choose This RT-qPCR Kit?

This one-step RT-qPCR solution brings multiple benefits to the lab bench:

• Streamlined Workflow: Reverse transcription and PCR happen in one tube, eliminating manual transfer steps.
• Reduced Contamination Risk: The UDG-dUTP anti-contamination system protects your results from aerosolized amplicon contamination.
• Time-Saving Protocols: Compatible with both standard and fast cycling conditions to suit your schedule.
• Multiplex Ready: Easily supports multiple primer-probe sets for multiplex PCR applications.

It’s a wholesome solution crafted with care, aimed at easing the daily workload of researchers while boosting their confidence in experimental outcomes.

Expertly Engineered Composition

Each batch of RT-qPCR Master Mix includes:

• High-purity dNTP mix
• Optimized Mg²⁺ concentration
• Robust reverse transcriptase
• Hot-start Taq DNA polymerase (antibody-modified)
• RNase inhibitor
• UDG enzyme for contamination prevention

It’s available in formats for 100, 1000, or 5000 reactions, making it perfect for labs of all sizes. Shipped under cold conditions and stored at -25°C to -15°C, this mix is made to last.

How to Use the One-Step RT-qPCR Master Mix

Standard Setup (20 μL reaction):

• 5 μL 4× Master Mix
• 0.4 μL each of forward and reverse primers (10 μM)
• 0.2 μL probe (10 μM)
• Template RNA (1 pg to 1 μg)

• RNase-free water to 20 μL

Thermal Cycling Programs:

• Standard: 55°C for 15 min → 95°C for 1 min → 45 cycles (95°C 10s, 60°C 30s)
• Fast: 55°C for 5 min → 95°C for 1 min → 42 cycles (95°C 5s, 60°C 15s)

This RT-qPCR kit is designed for flexibility and efficiency. Whether you're using a standard or fast protocol, your results will remain accurate and reproducible.

Tips for Better Primers and Probes

Designing primers and probes correctly is essential for multiplex RT-qPCR success. Here are a few user-friendly tips:

Primer Design:

• Length: 18–30 bases
• GC Content: 40–60%
• Tm: Ideally 55–60°C
• Avoid self-complementarity and primer-dimer formation

Probe Design:

• Length: 18–30 bases
• Tm: 65–70°C
• Avoid 5' guanine and secondary structures
• Use HPLC-purified probes for maximum sensitivity
Good design ensures specificity, efficiency, and reproducibility—hallmarks of trustworthy PCR data.

Crafted with Care for the Scientific Community

The One-Step RT-qPCR Master Mix isn’t just a product—it’s a symbol of the care and precision Hzymes brings to the life sciences industry. Every detail, from formulation to packaging, is designed to help researchers focus on what really matters: making discoveries that improve lives.

Whether you’re working on infectious disease diagnostics, gene expression studies, or viral detection, this mix offers the performance and support you need.

Supporting You Beyond the Bench

Hzymes aims to be a world-class provider of specialty enzyme products and services. Behind every reaction, there’s a team dedicated to empowering your science with consistency, innovation, and integrity.

Got questions? Their responsive support team is always ready to help you get the most out of your experiments.

🌐 Visit: https://hzymesbiotech.com/ 
📧 Email: info@hzymes.com

Conclusion

In a world where science often moves at the speed of urgency, the One-Step Multiplex RT-qPCR Master Mix stands as a beacon of thoughtful innovation. It simplifies protocols, ensures reliable results, and offers peace of mind—all in one tube.

When your research matters, your tools should too.