PCR technology plays a crucial role in molecular biology due to its high sensitivity and accuracy. However, one major challenge is nucleic acid aerosol contamination, which can lead to false positive results and compromise research integrity. To address this issue, UDG enzyme is widely used for contamination control in PCR assays.

How UDG Enzyme Works

UDG (Uracil-DNA Glycosylase) catalyzes the hydrolysis of N-glycosidic bonds between the uracil base and the sugar-phosphate backbone in single-stranded or double-stranded DNA. This allows selective degradation of dU-containing PCR products without affecting the original DNA template. By effectively removing residual amplification products, UDG enzyme ensures highly accurate PCR results.

Types of UDG Enzymes

There are two primary types of UDG enzymes available in the market:

1.Ordinary UDG Enzymes 

Derived from Escherichia coli and Bacillus subtilis, these enzymes have residual activity after inactivation, which may lead to unexpected degradation.

2.Heat-sensitive UDG Enzymes 

Sourced from psychrophilic marine bacteria, these enzymes can be completely inactivated at relatively low temperatures, preventing unintended degradation.

Hzymes Heat-sensitive UDG Enzyme: A Game Changer

Hzymes Biotech has developed an innovative heat-sensitive UDG enzyme, designed to be completely inactivated at temperatures above 50°C. This feature eliminates residual enzymatic activity, ensuring accurate PCR amplification and preventing false positives due to contamination.

Key Features and Applications

Product Features:

High-temperature sensitivity – Irreversible inactivation above 50°C
Optimized contamination control – Effectively removes dU-containing products without affecting template DNA
Available in glycerol-free formulation – Compatible with freeze-drying systems

Applications:

PCR, qPCR, RT-PCR, RT-qPCR, RT-LAMP
Molecular diagnostics and forensic science

Environmental and food safety testing

Why Choose Hzymes Heat-sensitive UDG Enzyme?

1. Superior Digestion Efficiency

Compared to competitors A and B, Hzymes UDG enzyme exhibits better digestion of uracil-containing DNA, ensuring complete removal of contaminants.

2. Enhanced Heat Inactivation

Hzymes UDG enzyme is irreversibly inactivated after treatment at 50°C, eliminating residual activity and preventing post-PCR degradation, unlike standard UDG enzymes.

3. Stable Performance in PCR Systems

No adverse effects on dual and multiplex PCR systems

Consistent and reliable amplification results

4. Excellent Thermal Stability

Hzymes UDG enzyme remains stable even after 10 days of accelerated storage at 37°C, ensuring long shelf-life and reliability.

Product Information

Hzymes Biotech offers various formulations of heat-sensitive UDG enzyme to cater to different research and industrial needs. Proper storage under recommended conditions guarantees maximum enzyme activity.

Conclusion

For researchers and professionals looking for high-precision, contamination-free PCR solutions, Hzymes Biotech Heat-sensitive UDG Enzyme is the ideal choice. With superior heat inactivation, efficient DNA digestion, and stable performance, it ensures accurate, reliable, and reproducible PCR results.