Overview

Ultra Nuclease (GMP Grade) is a high-purity endonuclease enzyme designed to efficiently degrade all forms of DNA and RNA in bioprocessing and viral vector production. With a DMF filing (No. MF036352) and GMP-compliant manufacturing, it ensures exceptional reliability, safety, and regulatory readiness for biopharmaceutical and gene therapy workflows.

The enzyme hydrolyzes phosphodiester bonds in nucleotides, completely digesting free nucleic acids. Structurally, Ultra Nuclease consists of two subunits (~30 kDa each) with an isoelectric point (pI) of 6.85, offering balanced activity and stability under process conditions.

Key Applications

Efficient nucleic acid removal is critical in biomanufacturing, where residual DNA or RNA can affect product purity, safety, and downstream processing. Ultra Nuclease delivers superior performance across multiple applications:

Viral Vaccine and Vector Purification

Efficiently removes host-cell DNA and RNA from viral vaccines, adenovirus, AAV, and lentivirus production.

Cell and Gene Therapy Manufacturing

Supports GMP-grade purification of viral and non-viral gene therapy vectors, improving downstream efficiency and compliance.

Particle Pretreatment

Removes nucleic acids wound around viral particles, facilitating virus release and purification.

Viscosity Reduction

Eliminates nucleic acids in crude extracts, reducing viscosity and improving protein yield.

PBMC Storage Optimization

Reduces cell clumping in stored peripheral blood mononuclear cells, enhancing culture stability.

GMP Compliance and Quality Assurance

Ultra Nuclease is manufactured under GMP conditions in strict accordance with regulatory standards.

✅ No antibiotics used during production
✅ Free from TSE/BSE-risk materials
✅ No raw materials of animal origin
✅ Documented Drug Master File (DMF No. MF036352)

These assurances make it suitable for clinical-grade biopharmaceutical production and regulatory submissions worldwide.

Product Features

• High Specific Activity ≥ 1.0 × 10⁶ U/mg

• Excellent Stability — thermal and freeze-thaw resistant

• Batch Consistency — inter-batch CV < 5%

• Animal Origin-Free — ideal for sensitive therapeutic applications

• DMF-Filed — supports global regulatory compliance

Performance Data

Quality Data

Application

Compared under the same enzyme activity

Comparison of plasmid digestion ability under same enzyme activity across different manufacturers: Plasmid digestion ability: Hzymes’s Super Ultra Nuclease > Competitor B > Competitor A.

Compared at the same volume

Comparison of plasmid digestion ability at the same volume across different manufacturers: Plasmid digestion ability: Hzymes’s Super Ultra Nuclease > Competitor B > Competitor A.

Viscosity reduction in bacterial suspension

Why Choose Hzymes Ultra Nuclease (GMP Grade)?

•        GMP-Certified Production ensures batch-to-batch consistency

•        DMF-Filed (MF036352) for smooth regulatory filing

•        Cost-Effective and Scalable for commercial biomanufacturing

•        Validated in Viral Vector and Vaccine Production

•        Reliable Supply Chain from a trusted global enzyme manufacturer

Conclusion

As nucleic acid contamination control becomes a key quality requirement in vaccine, gene therapy, and biopharmaceutical manufacturing, Hzymes Ultra Nuclease (GMP Grade) delivers a robust, regulatory-ready solution. Its high activity, excellent stability, and GMP compliance make it the preferred choice for next-generation bioprocessing workflows.