Introduction

Glycerol-3-phosphate oxidase (G3PO) is a key enzyme in clinical chemistry and biochemical analysis. It catalyzes the oxidation of glycerol-3-phosphate (G3P) to dihydroxyacetone phosphate (DHAP) and hydrogen peroxide (H₂O₂), playing a central role in enzymatic triglyceride assays.

At Hzymes Biotechnology, our high-purity microbial G3PO (Catalog No. HH1103) delivers exceptional stability, reproducibility, and assay performance, supporting diagnostic reagent manufacturers and research laboratories worldwide.

Enzymatic Properties of Hzymes G3PO

Key Advantages of Hzymes G3PO

1. Exceptional Thermal Stability

Enzyme activity retention. After 30 min incubation at different temperatures (A); After 40min incubation at 50°C（B）.

After reconstitution of the lyophilized powder, the enzyme solution was prepared. The retained activity of Hzymes G3PO after incubation at various temperatures (25-45°C for 30 min, and 50°C for 40 min) was higher than that of other commercial G3PO enzymes.

2. Wide pH Stability Range

Relative enzyme activity at different pH values.

Hzymes G3PO exhibits a wider pH stability range, maintaining over 80% relative activity within the pH range of 5.5 to 9.5.

3. Resistance to Surfactants and Metal Ions

Hzymes G3PO is resistant to a wide range of surfactants, metal ions, and preservatives.

4. Low Batch-to-Batch Variation

The variation in specific activity and thermal stability between batches is less than 5%. Low batch-to-batch variation ensures reproducible experimental results.

Applications

• Enzymatic Triglyceride Assays – Core enzyme for multi-step reactions involving lipoprotein lipase, glycerol kinase, and G3PO, enabling accurate quantification of serum triglycerides.
• Clinical Chemistry Reagents – Integration into enzymatic colorimetric assays and automated analyzer reagents.
• Biochemical Research – Investigation of glycerol metabolism, oxidative pathways, and cellular energy studies.

Why Choose Hzymes G3PO

l  High enzyme activity and purity

l  Broad pH and temperature stability

l  Resistance to surfactants, preservatives, and metal ions

l  Proven consistency across batches

l  Flexible OEM and bulk supply for diagnostic manufacturers

About Hzymes Biotechnology

Hzymes Biotechnology is a global supplier of diagnostic enzymes, antibodies, and molecular biology reagents, providing end-to-end solutions for clinical chemistry, immunodiagnostics, and molecular diagnostics.

Our enzyme portfolio covers oxidases, dehydrogenases, kinases, and transferases optimized for high stability, batch consistency, and large-scale OEM production.