Background

Nowadays, diabetes is spreading rapidly and even affecting younger people. Many young individuals have developed diabetes due to poor dietary habits.

According to the latest statistics on diabetes in China, by 2023, there are approximately 141 million people aged 20 to 79 living with diabetes in the country.

Self-monitoring of blood glucose is a critical component of diabetes management, aiming to promote better self-care.

Most self-monitoring devices on the market use portable glucose meters, which are mainly based on two methods: glucose oxidase method (GOD) and glucose dehydrogenase method (GDH).
The GOD method is affected by oxygen interference and is unsuitable for patients with unstable blood oxygen levels. The GDH method is not influenced by oxygen, but the enzyme has poor specificity, making it prone to interference from sugars like maltose, galactose, and xylose in the blood sample.

Newly Upgraded Glucose Dehydrogenase (GDH-FAD Type)

Hzymes has long been dedicated to the development of specialty enzymes and continuous innovation. The newly upgraded glucose dehydrogenase (GDH-FAD type) further enhances the quality of blood glucose test strips.

Features

Newly Upgraded Glucose Dehydrogenase

1. Enzyme sequence mutation enhances stability
   Through the introduction of a series of mutation points, enzyme stability has been significantly improved.
2. Optimized lyophilized formulation improves adaptability
   To reduce interference, a version without lyophilization protectants (only basic buffer solution included) has been developed. Both lyophilized and non-lyophilized versions are available to improve compatibility with various systems.

Outstanding Performance

1. Excellent substrate selectivity with strong anti-interference ability
   Almost no reaction with xylose or other sugars. When diabetic patients take or are injected with xylose-containing formulations, this helps reduce false glucose readings caused by sugar interference.
2. Wider pH range enhances reagent compatibility
   Performs excellently across a pH range of 2.0–10.0, making it suitable for more systems and application scenarios.
3. Strong chemical tolerance broadens formulation potential
   Tolerant to various chemical substances, with strong anti-interference capabilities in complex samples, and enables the development of more reagent formulations.

Table 2: Product Properties

Data

Enzyme sequence mutation improves enzyme stability

Thermostability has been enhanced, improving the performance of reagents and test strips.

Optimized lyophilized formulation enhances enzyme adaptability

To reduce interference from external substances, a version without lyophilization protectants has been specifically developed.

Excellent substrate selectivity improves anti-interference performance

Effectively excludes interference from xylose, maltose, mannose, galactose, and other sugars.

Wider pH range enhances reagent adaptability

The enzyme demonstrates excellent activity under extreme pH conditions.

Strong tolerance broadens formulation potential

Tolerant to various surfactants and metal ions; compared to similar products, it shows stronger anti-interference ability against Ag and Zn ions.

Table1: The enzyme dissolved in 50 mM K-phosphate buffer (pH6.0) was incubated with each chemical at 25'c for 1hr. Enzyme concentration: 50 U/mL.