Product Overview
Proteinase K (Liquid) is a highly stable serine protease with broad substrate specificity, engineered for efficient protein degradation in molecular biology and in vitro diagnostic workflows. It belongs to the subtilisin family and contains a conserved catalytic triad (Asp39–His69–Ser224), enabling robust proteolytic activity under a wide range of conditions.
This liquid formulation maintains strong enzymatic performance even in the presence of chaotropic agents and detergents such as guanidine hydrochloride and guanidine isothiocyanate, making it suitable for demanding nucleic acid extraction and purification processes.
Proteinase K (Liquid) is widely used in genetic testing, molecular diagnostics, and biopharmaceutical sample preparation, particularly in workflows requiring high-purity DNA and RNA recovery.
Key Features
High Enzymatic Activity
• Engineered through directed evolution for enhanced catalytic efficiency and protein digestion performance.
Strong Chaotropic Salt Tolerance
• Maintains activity in guanidine hydrochloride and guanidine isothiocyanate-containing buffers commonly used in nucleic acid extraction.
High Purity / Residue-Free
• Free from detectable DNase, RNase, and nickase contamination
• DNA residue < 5 pg/mg
• No detectable heavy metal ions
Reliable Supply Capability
• Annual production capacity up to 1,500 kg, ensuring stable supply for industrial-scale and diagnostic manufacturing needs
mNGS-Compatible Quality Option
• Available high-purity grade suitable for low-biomass and high-sensitivity molecular workflows
Applications
DNA & RNA Extraction
• Efficient degradation of proteins and nucleases during nucleic acid purification, improving DNA and RNA yield and quality.
Molecular Diagnostic Reagents
• Used in PCR, qPCR, RT-PCR, and other nucleic acid-based diagnostic workflows requiring high-purity templates.
Genetic Testing Kits
• Applied in sample pre-treatment and nucleic acid extraction steps in genetic diagnostic workflows.
Biopharmaceutical Processing
• Removal of protein impurities during purification of nucleic acid-based therapeutics and biological products.
Tissue & Cell Sample Preparation
• Facilitates efficient protein digestion to release nucleic acids and non-protein components from biological samples.
Performance Data
1. High Nucleic Acid Recovery Performance
2. Guanidine Salt Tolerance
• Maintains enzymatic activity in chaotropic environments
• Compatible with guanidine hydrochloride and guanidine isothiocyanate extraction systems
3. Nuclease-Free Purity
• DNase-free
• RNase-free
• Nickase-free
• Ensures integrity of extracted nucleic acids for downstream applications
4. Stability
• Demonstrates long-term stability under controlled storage conditions
• Suitable for industrial production and diagnostic kit integration
FAQ
Q1: What are the key features of Proteinase K (Liquid)?
A1: It is a stable protease with no DNase contamination. It is tolerant to high salt conditions and is suitable for protein degradation in nucleic acid sample preparation workflows.
What is the storage condition of Proteinase K (Liquid)?
It can be stored at 25°C for up to 3 years according to product specifications.
What makes Proteinase K suitable for molecular biology applications?
It maintains enzyme activity under relatively harsh conditions, including high salt environments, making it suitable for routine molecular biology and nucleic acid processing workflows.
