Product Overview
FS qPCR Master Mix (Liquid, Lyoph-Ready) is a ready-to-use qPCR reagent optimized for DNA detection in both singleplex and multiplex quantitative PCR assays. The formulation combines a specialized qPCR buffer with enzyme mix and supports probe-based real-time PCR applications.
The master mix incorporates a UDG-dUTP contamination prevention system, which effectively minimizes the risk of carryover contamination caused by previous PCR amplification products. In addition, it is compatible with both standard and fast qPCR cycling protocols, helping reduce assay turnaround time while maintaining reliable amplification performance.
Designed for liquid formulations and lyophilized assay development, FS qPCR Master Mix is suitable for freeze-dried PCR reagent manufacturing, molecular diagnostics development, and research applications requiring convenient storage and workflow flexibility.
Product Components
| Component | 100 rxn | 1,000 rxn | 10,000 rxn |
|---|---|---|---|
| 2×qPCR Buffer | 1.25mL | 12.5mL | 12.5mL×10 |
| 25×Enzymes Mix | 100 μL | 1mL | 10mL |
Key Features
UDG-dUTP Contamination Control
Integrated UDG-dUTP system eliminates carryover PCR contamination, reducing false-positive results and improving assay reliability.
Compatible with Lyophilization
Supports preparation of freeze-dried PCR formulations, making it suitable for lyophilized diagnostic reagent development and room-temperature transportation strategies.
Fast qPCR Amplification
Compatible with rapid thermal cycling programs, enabling significantly shortened PCR run times compared with conventional protocols.
Supports Singleplex and Multiplex Detection
Optimized for both single-target and multi-target DNA quantification assays using TaqMan probe chemistry.
Flexible Workflow Design
Can be used directly as a liquid qPCR master mix or incorporated into freeze-dried bead and pellet production workflows.
Applications
• Probe-based quantitative PCR (qPCR)
• DNA target detection and quantification
• Multiplex qPCR assay development
• Molecular diagnostic kit development
• Lyophilized PCR reagent manufacturing
• Pathogen detection assays
• Food safety testing
• Environmental monitoring
• Research and clinical laboratory applications
Performance Data
Rapid Performance: Ultra-rapid RNA Detection in 20 Minutes
For the HMD3916 system, the Ct value variation is approximately 0.5 Ct across standard, rapid and ultra-rapid protocols, enabling reliable ultra-rapid detection in 20 minutes.
Anti-Inhibition Performance: Direct Amplification with Release
When the HMD3916 system is combined with the HMD3508 release reagent for 20-minute ultra-rapid detection, it exhibits excellent anti-inhibition performance.
Storage Stability: Lyophilized Beads (60-day Accelerated Aging at 50°C)
FAQ
What are the advantages of FS qPCR Master Mix (Liquid, Lyoph-Ready)?
FS qPCR Master Mix is designed for both liquid PCR workflows and lyophilization-based product development. It provides excellent amplification performance, high sensitivity, and strong stability after freeze-drying, making it ideal for molecular diagnostic kit development and room-temperature storage applications.
Is FS qPCR Master Mix compatible with lyophilization?
Yes. FS qPCR Master Mix is optimized for lyophilization and can be used to develop stable dry PCR reagents. It maintains reliable amplification performance after the freeze-drying and rehydration processes, supporting the development of transport-friendly and shelf-stable diagnostic products.
What types of samples can FS qPCR Master Mix tolerate?
FS qPCR Master Mix demonstrates strong tolerance to common sample matrices and PCR inhibitors. It is suitable for amplification from various sample types, including swabs, blood, sputum, stool, and other clinical specimens, depending on the assay design and sample preparation method.
Can FS qPCR Master Mix be used for direct PCR applications?
Yes. FS qPCR Master Mix supports direct amplification workflows when combined with appropriate sample release or preparation methods. It is suitable for rapid molecular detection workflows requiring simplified sample processing.
What detection platforms are compatible with FS qPCR Master Mix?
FS qPCR Master Mix is compatible with commonly used real-time PCR platforms and can support singleplex and multiplex qPCR assays. It is suitable for developing diagnostic assays targeting various pathogens and biomarkers.
Does FS qPCR Master Mix support multiplex qPCR assays?
Yes. FS qPCR Master Mix provides high specificity and efficient amplification performance, supporting multiplex qPCR assay development with multiple targets in a single reaction.
Can FS qPCR Master Mix be used with UDG/dUTP contamination control systems?
Yes. FS qPCR Master Mix is compatible with dUTP/UDG-based carryover contamination prevention systems, helping reduce contamination risks while maintaining reliable amplification performance.
What applications are suitable for FS qPCR Master Mix?
FS qPCR Master Mix is suitable for a wide range of molecular diagnostic applications, including pathogen detection, infectious disease testing, screening assays, and other high-throughput qPCR workflows requiring stable and reliable reagent performance.
What is the difference between liquid and lyophilized formats of FS qPCR Master Mix?
The liquid format provides convenient preparation and optimization during assay development, while the lyophil-ready formulation enables conversion into dry reagents for improved transportation stability and longer shelf life. Both formats deliver consistent PCR amplification performance.
Can Hzymes provide customized solutions for diagnostic kit development?
Yes. Hzymes provides customized PCR enzyme and reagent solutions, including formulation optimization, lyophilization support, and assay development assistance to meet the requirements of molecular diagnostic manufacturers.
