Product Overview
FS RT-qPCR Master Mix (Liquid, Lyoph-Ready) is a lyophilization-ready one-step RT-qPCR reagent specifically designed for RNA detection and quantification. It supports both singleplex and multiplex real-time PCR assays using RNA templates and is suitable for molecular diagnostic assay development, infectious disease testing, and freeze-dried reagent manufacturing.
The master mix incorporates a UDG-dUTP contamination prevention system to effectively eliminate carryover contamination from previous amplification products, reducing the risk of false-positive results. In addition, the formulation is compatible with fast RT-qPCR protocols, enabling rapid RNA detection while maintaining reliable amplification performance.
Designed for both liquid and freeze-dried workflows, FS RT-qPCR Master Mix provides flexibility for the development of room-temperature-stable RT-qPCR products and point-of-care molecular testing solutions.
Key Features
Ultra-Rapid RNA Detection
Optimized for fast RT-qPCR workflows, enabling RNA detection in as little as 20 minutes, helping accelerate the time from sample processing to result reporting.
Lyophilization-Ready Formulation
Specifically designed for freeze-dried assay development, supporting the production of lyophilized RT-qPCR beads and pellets with convenient storage and transportation options.
UDG-dUTP Contamination Prevention
Integrated UDG-dUTP system minimizes carryover contamination from previous PCR products, improving assay accuracy and reliability.
Stress-Tolerant Direct Amplification
Compatible with direct amplification workflows following sample lysis using release reagents, demonstrating strong tolerance to inhibitors commonly encountered in crude samples.
User-Friendly Workflow
Supports one-step sample addition for complete rehydration of lyophilized beads, simplifying experimental procedures and reducing hands-on time.
Superior Stability
Lyophilized formulations maintain stable performance after 60 days of accelerated aging at 50°C, supporting ambient-temperature transportation and storage strategies.
Applications
• One-step RT-qPCR detection of RNA targets
• Viral RNA detection and quantification
• Infectious disease molecular diagnostics
• Multiplex RT-qPCR assay development
• Freeze-dried molecular diagnostic reagent development
• Point-of-care testing (POCT)
• Direct amplification workflows using release reagents
• Research and clinical laboratory applications
• Environmental and food safety RNA testing
Performance Data
Rapid Performance: Ultra-rapid RNA Detection in 20 Minutes
For the HMD3916 system, the Ct value variation is approximately 0.5 Ct across standard, rapid and ultra-rapid protocols, enabling reliable ultra-rapid detection in 20 minutes.
Anti-Inhibition Performance: Direct Amplification with Release
When the HMD3916 system is combined with the HMD3508 release reagent for 20-minute ultra-rapid detection, it exhibits excellent anti-inhibition performance.
Storage Stability: Lyophilized Beads (60-day Accelerated Aging at 50°C)
FAQ
Q1: What types of samples are compatible with FS RT-qPCR Master Mix?
A1: FS RT-qPCR Master Mix supports sensitive RNA detection workflows and is compatible with various sample types. It can be used with extracted nucleic acids and is suitable for applications requiring robust amplification performance.
Q2: Does FS RT-qPCR Master Mix support direct amplification of clinical samples?
A2: Yes. With optimized resistance to common sample inhibitors, FS RT-qPCR Master Mix can support direct amplification workflows when combined with appropriate sample pretreatment methods.
Q3: Which resistant RNA MIX and DNA MIX are available, and what types of samples can they tolerate?
A3: The recommended resistant RNA MIX is HMD6906, which supports direct amplification of swab and stool samples, as well as extracted samples from swabs, blood and diarrhea specimens.
The recommended resistant DNA MIX includes HMD3808, HMD6801 and HMD3819, which support direct amplification of samples such as swabs, blood and sputum.
