Product Overview
FS Hot Start Plus Taq DNA Polymerase (Antibody Modified) is a hot-start DNA polymerase designed for robust PCR performance under challenging sample conditions. The enzyme activity is blocked by a specific antibody at room temperature and rapidly activated during a short high-temperature incubation step, enabling controlled reaction initiation and improved amplification specificity.
This polymerase is engineered for PCR applications involving complex templates, including crude biological samples and inhibitor-containing extracts. It demonstrates strong resistance to common PCR inhibitors and is suitable for routine PCR, multiplex detection, and rapid amplification workflows.
FS Hot Start Plus Taq is compatible with standard PCR systems and supports applications requiring high sensitivity, specificity, and operational convenience, including premixed reagent development.
Key Features
Antibody-mediated hot-start control
The polymerase is inactivated at room temperature by a specific antibody and rapidly activated at 95°C, reducing non-specific amplification and improving reaction specificity.
Strong tolerance to PCR inhibitors
Performs reliably in the presence of common inhibitors such as whole blood components, humic acids, ethanol, and guanidine salts, supporting PCR from crude or minimally processed samples.
Fast extension capability
Supports rapid DNA synthesis, enabling efficient amplification of medium-length targets under shortened extension times, improving workflow efficiency in fast PCR protocols.
High sensitivity for low-copy detection
Enables stable amplification of low-abundance templates with good reproducibility, suitable for sensitive detection applications.
High specificity in multiplex PCR
Maintains clear and specific amplification in multiplex reactions, supporting multi-target detection with reduced non-specific products.
Compatible with dUTP/UDG systems
Demonstrates good tolerance to uracil-containing templates, supporting integration into UDG-based carryover contamination prevention workflows.
Premix system compatibility
Suitable for development of premixed PCR reagents, supporting simplified workflow design and improved operational consistency.
Applications
FS Hot Start Plus Taq DNA Polymerase is suitable for a wide range of PCR-based applications, including:
• Routine PCR amplification
• Direct PCR from crude or inhibitor-containing samples
• Multiplex PCR detection assays
• Fast PCR and rapid diagnostic workflows
• Low-copy target detection
• dUTP/UDG contamination control systems
• Premixed PCR reagent development
Performance Data
PCR inhibitor tolerance
FS Hot Start Plus Taq DNA Polymerase maintains stable amplification performance in the presence of common PCR inhibitors, including whole blood, nucleic acid release reagents (e.g., ethanol and guanidine hydrochloride), and humic acid.
In comparative evaluations under simulated clinical and environmental sample conditions, the enzyme demonstrated improved robustness, supporting reliable amplification from complex sample matrices such as blood, swabs, soil, and plant extracts.
Fast PCR extension performance
The enzyme supports efficient DNA synthesis under shortened extension times.
In evaluations using a 2 kb target fragment, FS Hot Start Plus Taq successfully supported amplification under rapid extension conditions, demonstrating suitability for time-efficient PCR workflows and rapid diagnostic applications.
Sensitivity for low-copy detection
In plasmid dilution experiments ranging from low to high copy numbers, FS Hot Start Plus Taq demonstrated reliable detection performance at low template concentrations.
The amplification results showed good linearity and reproducibility across dilution gradients, indicating suitability for sensitive detection applications.
Multiplex PCR performance
In multiplex PCR systems containing multiple target fragments, FS Hot Start Plus Taq maintained specific amplification across all expected targets.
The results indicated stable performance in multi-target detection assays with reduced non-specific amplification.
dUTP compatibility
The enzyme shows good compatibility with dUTP-containing reaction systems.
No significant changes in amplification performance were observed under different dUTP conditions, supporting its use in UDG-based contamination control workflows.
Premix system stability
FS Hot Start Plus Taq is compatible with premixed PCR systems.
Stability testing of premixed reaction systems stored under accelerated conditions showed no significant differences in amplification performance compared with freshly prepared controls, indicating suitability for kit development and diagnostic reagent manufacturing.
FAQ
What is the difference between FS HotStart Plus Taq and standard FS HotStart Taq?
FS HotStart Plus Taq is an upgraded version with enhanced inhibitor tolerance and improved amplification efficiency. It maintains hot-start control while offering better performance in challenging sample matrices.
What types of inhibitors can it tolerate?
It shows strong resistance to common PCR inhibitors such as blood-derived components, humic acids, and environmental contaminants, making it suitable for crude or minimally processed samples.
Can it be used for multiplex PCR?
Yes. FS HotStart Plus Taq is optimized for multiplex amplification, providing balanced target amplification and reducing bias between multiple primer pairs in the same reaction.
What are its main application scenarios?
It is suitable for complex template PCR, direct amplification from inhibitor-containing samples, multiplex PCR assays, and molecular diagnostics requiring robust performance.
