Product Overview
Multiplex qPCR Probe Kit U+ (Fast) is a probe-based real-time quantitative PCR (qPCR) reagent designed for rapid and multiplex nucleic acid detection. Featuring a fast amplification system, the kit can complete 42 PCR cycles within 40 minutes, helping reduce turnaround time while maintaining reliable amplification performance.
The kit includes a heat-labile Uracil-DNA-Glycosylase (UDG) contamination control system, which degrades uracil-containing carryover amplicons from previous reactions, minimizing the risk of false-positive results caused by aerosol contamination.
In addition to standard probe-based qPCR applications, the kit supports multiplex fluorescence detection and fluorescence melting curve analysis, providing flexibility for assay development and molecular diagnostic workflows.
Key Features
Fast qPCR Amplification
Optimized enzyme and buffer formulation enables rapid PCR cycling, completing 42 amplification cycles in approximately 40 minutes and supporting fast turnaround workflows.
Carryover Contamination Prevention
Contains heat-labile UDG in the enzyme mix to eliminate contamination from previous PCR products, improving assay reliability and reducing false-positive risks.
Multiplex Detection Compatibility
Supports multiplex fluorescent probe assays and is compatible with applications requiring simultaneous detection of multiple targets.
Fluorescence Melting Curve Analysis
Enables both amplification curve analysis and multi-color fluorescence melting curve analysis, supporting assay design strategies that combine probe detection with melting curve characterization.
Direct Amplification Capability
Demonstrated compatibility with direct amplification workflows using liquefied sputum samples processed with release reagents, helping simplify sample preparation procedures.
Applications
Multiplex qPCR Probe Kit U+ (Fast) is suitable for:
• Multiplex real-time PCR (qPCR) assays using probe detection chemistry
• Pathogen nucleic acid detection based on qPCR methodology
• Fluorescence melting curve analysis and genotyping assays
• Molecular diagnostic assay development
• Research laboratories and diagnostic reagent developers
• Applications requiring rapid PCR detection workflows
• Direct amplification workflows from appropriately processed biological samples
Performance Data
Rapid Direct Amplification from Liquefied Sputum Samples
HPV Genotype Detection by Melting Curve Analysis
For Research Use Only. Performance may vary depending on assay design, instrument platform, sample type, and experimental conditions.
FAQ
Q1: What types of samples can HMD6801 tolerate?
A1: HMD6801 is a resistant DNA MIX designed for multiplex qPCR applications. It supports direct amplification of challenging samples, including swabs, blood and sputum samples.
Q2: What are the advantages of HMD6801 in direct amplification applications?
A2: HMD6801 provides strong inhibitor tolerance and reliable amplification performance, helping maintain sensitivity and stability when working with complex biological samples.
Q3: Which resistant RNA MIX and DNA MIX are available, and what types of samples can they tolerate?
A3: The recommended resistant RNA MIX is HMD6906, which supports direct amplification of swab and stool samples, as well as extracted samples from swabs, blood and diarrhea specimens.
The recommended resistant DNA MIX includes HMD3808, HMD6801 and HMD3819, which support direct amplification of samples such as swabs, blood and sputum.
