Specification
Appearance | White to brownish amorphous powder,lyophilized |
Proteinpurity | ≥90%(from SDS-PAGE) |
Activity | ≥15 U/mg solid |
ATPase | ≤0.005% |
Catalase | ≤0.001% |
Glucoseoxidase | ≤0.01% |
Hexokinase | ≤0.01% |
Glycerolkinase | ≤0.01% |
Uricase | ≤0.01% |
Properties
EC number | 3.1.1.34 (Recombinant from microorganism) | |
Molecular weight | 47 kDa (SDS-PAGE) | |
Isoelectric point | 5.2 | |
Michaelis constants | 3.95×10-3M | |
Inhibitors | Ag+,Hg2+,Cr2+,Sn2+,Cu2+ | |
OptimumpH | 7.5 | Fig.1 |
Optimum temperature | 50℃ | Fig.2 |
pHstability | pH6.0-10.0(25℃,60min) | Fig.3 |
Thermal stability | Below50℃ (pH7.5,30min) | Fig.4 |
Storage stability | At least one year at 2~8℃ | Fig.5 |
Stabilizers | BSA |
Applications
This enzyme is used in diagnostic tests for the determination of triglycerides together with Glycerol kinase, and Glycerol-3-phosphate oxidase.
Unit definition
One unit is defined as the amount of enzyme which produces 1 μmole of glycerol per minute under the conditions described below.