Product Description
Instruction : This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Porcine trypsin antibody. Trypsin present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine trypsin antibody is added and binds to trypsin in the sample. After washing, HRP-Streptavidin is added and binds to the Biotinylated trypsin antibody. After incubation unbound HRP-Streptavidin is washed away. Then TMB substrate solution is added and catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of trypsin sample captured in plate. The absorbance is measured at 450 nm.
Product Information
Product Name | Cat. No | Size |
Trypsin Residue Detection Kit (ELISA) | HBP000211 | 96T |
Transportation and storage
1. For unused kit: The whole kit could be stored at 2~8℃ in shelf life. Strong light should be avoided for storage stability.
2. For used kit: Once the microplate is opened, please cover unused wells with plate sealer and return to the foil pouch containing the desiccant pack, zip-seal the foil pouch and return to 2~8℃ as soon as possible after use. Other reagents should be returned to 2~8℃ as soon as possible after use.