Product Description
PNGase F is the most effective enzymatic method for removing almost all N-linked oligosaccharides from glycoproteins. PNGase F is an amidase, which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins. It is very important for effective process control to obtain the accurate distribution of N-glycans in a short time. Generally, it takes several hours to hydrolyze and release the N-glycans of the antibody with PNGase F, and then carry out glycan derivatization and HPLC or mass spectrometry analysis. Especially, incomplete deglycosylation can lead to biased results, which will not represent the correct composition of the therapeutic antibody. PNGase F-Fast is an improved reagent, which can release the N-glycans of therapeutic antibody in a few minutes. All N-glycans can be released quickly without bias, and are ready for downstream chromatography or mass spectrometry analysis, so as to determine the glycoprofile of antibodies quickly.
Product Information
| Product Name | Cat. No | Size |
| PNGase F-Fast (Glycerol Free) | HBP003004 | 50μL |
Applications
The enzyme is used for rapid deglycosylation of glycosylated antibody, and the purified sugar chain can be used for subsequent mass spectrometry to identify the glycotype of antibody
Storage condition
Transport Condition: -25~-15℃
Storage condition: -25~-15℃；the freezing and thawing times of the product are less than 5 times. It is recommended to store the product in small doses after thawing for the first time. After thawing for short term use, it can be stored at 2~8℃, and the recommended time is not more than 30 days.
