Specification
| Appearance | Yellow amorphous powder, lyophilized |
| Protein purity | ≥90% (from SDS-PAGE) |
| Activity | ≥8 U/mg solid |
| Catalase | ≤0.001% |
| Cholesterol esterase | ≤0.001% |
| Glucose oxidase | ≤0.01% |
| ATPase | ≤0.005% |
Properties
| EC number | 1.1.3.6 (Recombinantfrom microorganism) | |
| Molecular weight | 60kDa (SDS-PAGE) | |
| Isoelectric point | 5.6 | |
| Michaelis constants | 3.3×10-4M (Cholesterol) | |
| Inhibitors | SDS,Ag+,Zn2+,Hg2+ | |
| Optimum pH | 7.0 | Fig.1 |
| Optimum temperature | 50℃ | Fig.2 |
| pH stability | pH5.5-10(25℃,16h) | Fig.3 |
| Thermal stability | Below 40℃(pH7.0,30min) | Fig.4 |
| Storage stability | At least one year at-25~-15℃ | Fig.5 |
| Stabilizers | BSA and non-ionic surfactants | |
Applications
This enzyme is useful for enzymatic determination of total cholesterol, HDL-C, and LDL-C when coupled with cholesterol esterase.
Unit definition
One unit is defined as the amount of enzyme which generates 1 μmol of H2O2 per minute at 37℃ under the conditions described below.
