Preparation and specification
| Appearance | Yellow amorphous powder, lyophilized |
| Purity(SDS-PAGE) | ≥90.0% |
| Enzyme powder specific activity | ≥450.0 U/mg |
| Glucose dehydrogenase (NAD) | ≤0.02% |
| Hexokinase | ≤0.02% |
| α-glucosidase | ≤0.02% |
Properties
| EC number | 1.1.5.9 (Recombinant from microorganism) | |
| Molecular weight | 66 kDa (SDS-PAGE) | |
| Isoelectric point | 5.04 | |
| Michaelis constants | 7.45 ×10-2 M (D-glucose) | |
| Inhibitors | Ag+, Cu2+, SDS | |
| Optimum pH | 7.0 | Fig. 1 |
| Optimum temperature | 50-55 °C | Fig. 2 |
| pH stability | pH 5.0 - 9.0 (25 °C, 16 h) | Fig. 3 |
| Thermal stability | Stable at 50 °C and blow (pH 7.0, 30 min) | Fig. 4 |
| Storage stability | At least one year at -25～-15 ℃ | Fig. 5 |
Applications
This enzyme is useful for clinical analysis of D-glucose determination in blood glucose meter.
Unit definition
One unit (U) is defined as the amount of enzyme which generates 1 μmol of DCIP per minute at 37 °C under the conditions described below.
