In life science research, the accuracy and efficiency of RNA detection are critical. Agilent's Fragment Analyzer series leverages automated parallel capillary electrophoresis to provide reliable nucleic acid quality control. Amid today's complex international trade environment, tariffs have become a major concern for many researchers. The Hzymes RNA Detection Kit, designed for compatibility with Agilent 5200 and related systems, offers a domestic alternative that delivers "import quality at local prices"—a smooth and economical transition for your laboratory.
Capillary Gel Electrophoresis
In capillary gel electrophoresis, the gel matrix is housed within the capillary and serves as a support
for electrophoresis detection. The porous nature of the gel acts like a molecular sieve, allowing separation based
on molecular size. Its high viscosity minimizes solute diffusion, producing sharp peaks and achieving the highest
column efficiency in CE.
This product includes denaturants that unfold secondary structures in single-stranded RNA, ensuring the RNA fragments remain in a denatured state. RNA fragments are introduced at the anode by electrokinetic or pressure injection and then rapidly separated by molecular weight under a high-voltage electric field. This enables accurate sizing and quantitative analysis of nucleic acid components.
1. Reagent Composition (One-to-One Replacement, Seamless Integration)
Component |
HBP005011-2 |
Agilent DNF-471 RNA (15nt) |
RNA Separation Gel |
250 mL |
RNA Separation Gel |
2× RNA Inlet Buffer |
250 mL |
5× 930 dsDNA Inlet Buffer |
5× Conditioning Solution |
100 mL |
5× Capillary Conditioning Soln, RT |
RNA Dilution Marker (15nt) |
3 vials, 4 mL each |
RNA Diluent Marker (15 nt) |
Blank Solution |
1 vial, 8 mL |
BF-25 Blank Solution |
Intercalating Dye |
1 vial, 30 μL |
Intercalating Dye |
RNA Ladder |
5 vials, 20 μL each |
RNA Ladder |
2. Performance (Stable and Reproducible)
Figure 1: RNA 6000 Ladder Electropherogram
Figure 2: RNA 6000 Ladder Reproducibility
Figure 3: mRNA Sample Electropherogram
Figure 4: Total RNA Sample Electropherogram
Figure 5: Standard Curve
3. A Perfect Alternative to Imported Kits
Sample Name |
Length |
Detection Kit |
Integrity-1 |
Integrity-2 |
Integrity-3 |
Integrity-4 |
Average Integrity |
CV |
Sample-1 |
9000 nt |
Imported Kit |
79.90% |
79.60% |
79.70% |
78.10% |
79.33% |
0.90% |
|
|
Hzymes |
74.40% |
75.40% |
76.10% |
74.70% |
75.15% |
0.88% |
Sample-2 |
9000 nt |
Imported Kit |
76.80% |
77.50% |
76.50% |
76.80% |
76.90% |
0.48% |
|
|
Hzymes |
76.90% |
77.00% |
77.10% |
77.10% |
77.90% |
0.11% |
Sample-3 |
400 nt |
Imported Kit |
86.20% |
85.00% |
85.40% |
85.10% |
85.43% |
0.55% |
|
|
Hzymes |
84.80% |
85.60% |
83.90% |
85.60% |
84.98% |
0.83% |
Hzymes RNA Detection Kit (Capillary Electrophoresis Method)
Thanks to in-house production of key raw materials, Hzymes offers stable supply and pricing—unaffected
by international tariffs. Choosing Hzymes means choosing efficiency, precision, and value. Let us support your
scientific journey with dependable RNA detection solutions.
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Service Hotline: +86 400-808-5320
Large-scale production base: Building 6, Precision Medical Industry Base, Wuhan, China
Logistics & Supply Chain Center:417 Main St, Little Rock, AR 72201. United States.
Global Marketing Center: Hzymes Building, Fengxian District, Shanghai, China.
Service Hotline: +86 400-808-5320
Large-scale production base: Building 6, Precision Medical Industry Base, Wuhan, China.
Logistics & Supply Chain Center:417 Main St, Little Rock, AR 72201. United States.
Global Marketing Center: Hzymes Building, Fengxian District, Shanghai, China.
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