Introduction: The Essential Role of Glycan Analysis
In the dynamic world of life sciences, the understanding of N-glycans—complex carbohydrates attached to proteins—plays a vital role in therapeutic development, disease diagnostics, and immunological research. Glycoproteins, such as Immunoglobulin G (IgG), are particularly significant due to their biological activity being directly influenced by their glycosylation patterns.
To simplify and enhance this essential field, Hzymes offers a powerful solution: the HBP003120 and HBP003121 N-Glycan Analysis Kits. These kits provide researchers with a reliable, fast, and reproducible method for releasing, labeling, purifying, and analyzing N-glycans from glycoproteins.

Kit Overview: HBP003120 vs. HBP003121

Product	Samples/Kit	Ideal For
HBP003120	24 samples	Small-scale studies or pilot experiments
HBP003121	96 samples	High-throughput workflows or full-scale projects

Each kit includes four modular components:
• Glycan Release Module (includes PNGase F enzyme and buffer)
• Labeling Module (for fluorescent tagging)
• Purification Module (96-well plate-based SPE)
• Collection Module (reaction tubes, caps, collection plate, waste tray)

Step-by-Step Workflow: From Sample to Signal
1. Fast Glycan Release
The process begins by denaturing glycoproteins in a mild buffer and applying the PNGase F enzyme, which efficiently cleaves N-glycans at 50°C within 5 minutes. This rapid step is optimized for IgG but is compatible with a range of glycoproteins.
2. Fluorescent Labeling
Next, the released glycans are labeled with a proprietary fluorophore dissolved in anhydrous DMF. This step enhances the sensitivity of detection for downstream analysis and takes just 5 minutes at room temperature.
3. Solid Phase Extraction (SPE) Purification
To eliminate excess labeling reagents and isolate clean glycan profiles, the kit includes a 96-well plate system compatible with positive or negative pressure systems. A series of water, acetonitrile, and formic acid-based washes ensures high purity.
4. HILIC-FLR Analysis
Final analysis is carried out using Hydrophilic Interaction Liquid Chromatography (HILIC) with Fluorescence Detection (FLR). The suggested configuration uses:
• Column: ACQUITY UPLC® Glycan BEH Amide (2.1 x 150 mm)
• Mobile Phase A: 50 mM ammonium formate (pH 4.4)
• Mobile Phase B: 100% acetonitrile
• Detection: EX 265 / EM 425 nm

Why Choose HBP003120/HBP003121?
•  Fast Results – From sample prep to analysis in under 2 hours
•  High Sensitivity – Fluorescent labeling ensures excellent detection
•  Modular Design – Flexible workflow with easy scalability
•  Optimized for IgG – Validated with monoclonal antibodies
•  Reliable Purification – SPE plates reduce background and enhance accuracy
•  Friendly to All Labs – Compatible with common lab equipment

Essential Equipment Checklist
To get started, you’ll need:
• Acetonitrile (LC-MS grade), ultrapure water
• Heating module or dry bath (90°C and 50°C)
• SPE vacuum or pressure manifold
• Vortex mixer and pipettes
• Formic acid, ammonium formate buffer

Storage & Handling Tips
To ensure the best results, follow these storage conditions:
• PNGase F enzyme: -25 to -15°C, avoid repeated freeze-thaw cycles
• Labeling reagents: 2-8°C, store in the dark
• SPE plates and plasticware: Room temperature, keep dry and sealed

Conclusion: Making Glycan Science Accessible and Effective
The HBP003120 and HBP003121 N-Glycan Analysis Kits bring together speed, accuracy, and ease-of-use in one streamlined platform. Whether you are exploring therapeutic glycoproteins or advancing biomarker discovery, these kits offer a powerful yet approachable solution to N-glycan profiling.
With thoughtful design, validated reagents, and straightforward protocols, Hzymes empowers researchers to unlock the hidden world of glycans—one sample at a time.