In the ever-evolving world of scientific research, certain tools quietly stand behind the brightest breakthroughs. One such unsung hero is the APS-5 Chemiluminescence Substrate—a light yellow powder that plays a crucial role in immunoassay diagnostics. With its remarkable sensitivity, stability, and ease of use, APS-5 supports researchers in revealing the invisible, one glowing reaction at a time.

What Is APS-5 Chemiluminescence Substrate?
APS-5 is a high-performance chemiluminescence substrate developed for use in immunoassays involving alkaline phosphatase (ALP). This light-yellow powder might seem unassuming at first glance, but its capabilities are far-reaching. Designed to be stored at -25°C to -15°C, it can remain stable for over two years when protected from light.
Whether you're a seasoned scientist or a laboratory technician, APS-5 stands as a reliable partner in your workflow, especially in tests requiring sensitive and accurate ALP detection.

How APS-5 Works: The Science of Light

At the heart of APS-5’s function lies a beautifully simple principle: when catalyzed by alkaline phosphatase, APS-5 releases protons that generate a chemiluminescent signal. The amount of light emitted is directly proportional to the concentration of ALP present, allowing for highly accurate quantitative measurements.
This reaction is not only scientifically precise—it’s also fast and efficient. Within seconds, the chemiluminescence reaches its peak and remains stable, enabling reliable data collection.

Key Benefits of Using APS-5

APS-5 has quickly become a favorite in labs for good reason. Here are the standout benefits that make it a top choice for chemiluminescent assays:

1. Ultra-High Sensitivity

APS-5 can detect ALP concentrations as low as 1×10⁻¹⁹ mol (0.01 pg). This allows scientists to identify even the smallest presence of enzyme activity, enhancing diagnostic accuracy.

2. Fast and Stable Signal

The reaction completes quickly, reaching a plateau within seconds. The signal remains stable for an extended period, giving users flexibility in timing without compromising reliability.

3. Wide Dynamic Range

From 50 pg/mL to 50,000 pg/mL, APS-5 accommodates a wide range of ALP concentrations, making it suitable for various experimental needs.

4. Room Temperature Friendly

APS-5 functions well between 22°C and 35°C, eliminating the need for strict temperature control. This makes it especially useful in standard laboratory environments.

5. Long Shelf Life

When stored at the recommended conditions and shielded from light, APS-5 remains effective for over two years, reducing waste and ensuring consistent availability.
Proper Handling for Best Results
To get the most from APS-5, follow a few simple but important guidelines:
• Allow to equilibrate to room temperature (22–35°C) before use.
• Avoid exposure to bright light, which may degrade its performance.
• Do not refreeze after the substrate is dissolved.

These small acts of care ensure the reagent performs at its peak—every time.

Backed by Quality Assurance
APS-5 is manufactured with attention to detail and scientific rigor. Every batch is tested to ensure it meets high standards. For complete quality specifications, users can refer to the Certificate of Analysis included with the product.

This commitment to quality makes APS-5 not just a tool, but a trusted ally in precision diagnostics and research.

Conclusion: Empowering Science Through Light
APS-5 Chemiluminescence Substrate is more than just a reagent—it's a reflection of how innovation and simplicity can come together to support brilliant science. With unmatched sensitivity, a wide detection range, and dependable performance, APS-5 helps researchers illuminate new discoveries, one molecule at a time.
In a world that depends on accurate data and dependable tools, APS-5 shines quietly but powerfully—lighting the path to discovery.